Stored whole blood transfusion initiates serum amyloid A activation monitored by real-time dynamic imaging.

ABSTRACT:

Background

Transfusion of stored whole blood (SWB) to resuscitate severe traumatic haemorrhage patients in military operations and civilian emergency centres is being increasingly used in routine practice. It has been well established that transfusion of red blood cells (RBCs) after prolonged storage has harmful effects, mainly mediated by inflammation. Whether the side effects of inflammation are brought about by SWB transfusion remains unclear.

Materials and methods

A hepatocyte SAA (serum amyloid A) specific reporter mouse that facilitated non-invasive imaging of hepatocyte SAA expression was used to evaluate acute inflammation and acute-phase reaction after the transfusion of SWB or components separated from end-storage whole blood. The whole blood of C57BL/6 donor mouse

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9918385 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature