Spiders' digestive system as a source of trypsin inhibitors: functional activity of a member of atracotoxin structural family.

ABSTRACT: Spiders are important predators of insects and their venoms play an essential role in prey capture. Spider venoms have several potential applications as pharmaceutical compounds and insecticides. However, transcriptomic and proteomic analyses of the digestive system (DS) of spiders show that DS is also a rich source of new peptidase inhibitor molecules. Biochemical, transcriptomic and proteomic data of crude DS extracts show the presence of molecules with peptidase inhibitor potential in the spider Nephilingis cruentata. Therefore, the aims of this work were to isolate and characterize molecules with trypsin inhibitory activity. The DS of fasting adult females was homogenized under acidic conditions and subjected to heat treatment. After that, samples were submitted to ion exchange batch a

SUBMITTER: Neto OBS 

PROVIDER: S-EPMC9918498 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature