Co-dependent regulation of p-BRAF and potassium channel KCNMA1 levels drives glioma progression.

ABSTRACT: BRAF mutations have been found in gliomas which exhibit abnormal electrophysiological activities, implying their potential links with the ion channel functions. In this study, we identified the Drosophila potassium channel, Slowpoke (Slo), the ortholog of human KCNMA1, as a critical factor involved in dRaf^GOF glioma progression. Slo was upregulated in dRaf^GOF glioma. Knockdown of slo led to decreases in dRaf^GOF levels, glioma cell proliferation, and tumor-related phenotypes. Overexpression of slo in glial cells elevated dRaf expression and promoted cell proliferation. Similar mutual regulations of p-BRAF and KCNMA1 levels were then recapitulated in human glioma cells with the BRAF mutation. Elevated p-BRAF and KCNMA1 were also observed in HEK293T cells upon

SUBMITTER: Xie S 

PROVIDER: S-EPMC9918570 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature