Radiosynthesis and Evaluation of a C-11 Radiotracer for Transient Receptor Potential Canonical 5 in the Brain.

ABSTRACT:

Purpose

TRPC5 belongs to the mammalian superfamily of transient receptor potential (TRP) Ca²⁺-permeable cationic channels and it has been implicated in various CNS disorders. As part of our ongoing interest in the development of a PET radiotracer for imaging TRPC5, herein, we explored the radiosynthesis, and in vitro and in vivo evaluation of a new C-11 radiotracer [¹¹C]HC070 in rodents and nonhuman primates.

Procedures

[¹¹C]HC070 was radiolabeled utilizing the corresponding precursor and [¹¹C]CH₃I via N-methylation protocol. Ex vivo biodistribution study of [¹¹C]HC070 was performed in Sprague-Dawley rats. In vitro autoradiography study was conducted for the rat brain sections to characterize the radiotracer d

SUBMITTER: Yu Y 

PROVIDER: S-EPMC9918595 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature