Identifying STEDable BF₂-Azadipyrromethene Fluorophores.

ABSTRACT: BF₂-azadipyrromethenes are highly versatile fluorophores used for cellular and in vivo imaging in the near-infrared and far-red regions of the spectrum. As of yet, their use in conjunction with super-resolution imaging methodologies has not been explored. In this report, a series of structurally related BF₂-azadipyrromethenes has been examined for their suitability for use with stimulated emission depletion (STED) nanoscopy. The potential for STED imaging was initially evaluated using aqueous solutions of fluorophores as an effective predictor of fluorophore suitability. For live cell STED imaging in both 2D and 3D, several far-red emitting BF₂-azadipyrromethenes were successfully employed. Image resolution below the diffraction limit of a confocal microsco

SUBMITTER: Curtin N 

PROVIDER: S-EPMC9919209 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature