Neuroprotection by Skimmianine in Lipopolysaccharide-Activated BV-2 Microglia.

ABSTRACT: Skimmianine is a furoquinoline alkaloid which is found in the Zanthoxylum genus and also in other plants of the Rutaceae family. This study evaluated the effects of skimmianine on the production of pro-inflammatory mediators in LPS-activated BV-2 microglia. Cultured BV-2 cells were treated with skimmianine (10, 20 and 30 μM), followed by stimulation with LPS (100 ng/mL). Levels of TNFα and IL-6 in cell supernatants were measured using ELISA, while NO and PGE₂ levels were evaluated with Griess assay and EIA, respectively. Western blotting was used to determine the protein expression of iNOS, COX-2, phospho-p65 and phospho-IκBα. Results showed that Skimmianine reduced LPS-induced elevated the secretion of TNFα, IL-6, NO, and PGE₂, as well as the increased protein

SUBMITTER: Ogunrinade FA 

PROVIDER: S-EPMC9920223 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature