An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants.

ABSTRACT: Protein targeting is essential in eukaryotic cells to maintain cell function and organelle identity. Signal peptides are a major type of targeting sequences containing a tripartite structure, which is conserved across all domains in life. They are frequently included in recombinant protein design in plants to increase yields by directing them to the endoplasmic reticulum (ER) or apoplast. The processing of bacterial signal peptides by plant cells is not well understood but could aid in the design of efficient heterologous expression systems. Here we analysed the signal peptide of the enzyme PmoB from methanotrophic bacteria. In plant cells, the PmoB signal peptide targeted proteins to both mitochondria and the ER. This dual localisation was still observed in a mutated version of the signal

SUBMITTER: Spatola Rossi T 

PROVIDER: S-EPMC9920398 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature