One-Step Oxidation of Orange Peel Waste to Carbon Feedstock for Bacterial Production of Polyhydroxybutyrate.

ABSTRACT: Orange peels are an abundant food waste stream that can be converted into useful products, such as polyhydroxyalkanoates (PHAs). Limonene, however, is a key barrier to building a successful biopolymer synthesis from orange peels as it inhibits microbial growth. We designed a one-pot oxidation system that releases the sugars from orange peels while eliminating limonene through superoxide (O₂^{• -}) generated from potassium superoxide (KO₂). The optimum conditions were found to be treatment with 0.05 M KO₂ for 1 h, where 55% of the sugars present in orange peels were released and recovered. The orange peel sugars were then used, directly, as a carbon source for polyhydroxybutyrate (PHB) production by engineered Escherichia coli. Cell growth was

SUBMITTER: Davaritouchaee M 

PROVIDER: S-EPMC9920450 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature