Mobility of mPing and its associated elements is regulated by both internal and terminal sequences.

ABSTRACT:

Background

DNA transposable elements are mobilized by a "cut and paste" mechanism catalyzed by the binding of one or more transposase proteins to terminal inverted repeats (TIRs) to form a transpositional complex. Study of the rice genome indicates that the mPing element has experienced a recent burst in transposition compared to the closely related Ping and Pong elements. A previously developed yeast transposition assay allowed us to probe the role of both internal and terminal sequences in the mobilization of these elements.

Results

We observed that mPing and a synthetic mPong element have significantly higher transposition efficiency than the related autonomous Ping and Pong elements. Systematic mutation of the internal sequences of both mPing and mPong identified multipl

SUBMITTER: Redd PS 

PROVIDER: S-EPMC9921582 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature