The Effects of Titanium Dioxide Nanoparticles on Osteoblasts Mineralization: A Comparison between 2D and 3D Cell Culture Models.

ABSTRACT: Although several studies assess the biological effects of micro and titanium dioxide nanoparticles (TiO₂ NPs), the literature shows controversial results regarding their effect on bone cell behavior. Studies on the effects of nanoparticles on mammalian cells on two-dimensional (2D) cell cultures display several disadvantages, such as changes in cell morphology, function, and metabolism and fewer cell-cell contacts. This highlights the need to explore the effects of TiO₂ NPs in more complex 3D environments, to better mimic the bone microenvironment. This study aims to compare the differentiation and mineralized matrix production of human osteoblasts SAOS-2 in a monolayer or 3D models after exposure to different concentrations of TiO₂ NPs. Nanoparticles were

SUBMITTER: de Souza Castro G 

PROVIDER: S-EPMC9921996 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature