Oxytocin protective effects on zebrafish larvae models of autism-like spectrum disorder.

ABSTRACT:

Objectives

Autism is a complicated neurodevelopmental disorder characterized by social interaction deficiencies, hyperactivity, anxiety, communication disorders, and a limited range of interests. The zebrafish (Danio rerio) is a social vertebrate used as a biomedical research model to understand social behavior mechanisms.

Materials and methods

After spawning, the eggs were exposed to sodium valproate for 48 hr, after which the eggs were divided into eight groups. Except for the positive and control groups, there were six treatment groups based on oxytocin concentration (25, 50, and 100 μM) and time point (24 and 48 hr). Treatment was performed on days 6 and 7, examined by labeling oxytocin with fluorescein-5-isothiocyanate (FITC) and imaging with confocal microscopy

SUBMITTER: Rahmati-Holasoo H 

PROVIDER: S-EPMC9922369 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature