Splashed E-box and AP-1 motifs cooperatively drive regeneration response and shape regeneration abilities.

ABSTRACT: Injury triggers a genetic program that induces gene expression for regeneration. Recent studies have identified regeneration-response enhancers (RREs); however, it remains unclear whether a common mechanism operates in these RREs. We identified three RREs from the zebrafish fn1b promoter by searching for conserved sequences within the surrounding genomic regions of regeneration-induced genes and performed a transgenic assay for regeneration response. Two regions contained in the transposons displayed RRE activity when combined with the -0.7 kb fn1b promoter. Another non-transposon element functioned as a stand-alone enhancer in combination with a minimum promoter. By searching for transcription factor-binding motifs and validation by transgenic assays, we revealed that the cooperation of E

SUBMITTER: Tamaki T 

PROVIDER: S-EPMC9922731 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature