The store-operated Ca²⁺ channel Orai1α is required for agonist-evoked NF-κB activation by a mechanism dependent on PKCβ2.

ABSTRACT: Store-operated Ca²⁺ entry is a ubiquitous mechanism for Ca²⁺ influx in mammalian cells that regulates a variety of physiological processes. The identification of two forms of Orai1, the predominant store-operated channel, Orai1α and Orai1β, raises the question whether they differentially regulate cell function. Orai1α is the full-length Orai1, containing 301 amino acids, whereas Orai1β lacks the N-terminal 63 amino acids. Here, using a combination of biochemistry and imaging combined with the use of human embryonic kidney 293 KO cells, missing the native Orai1, transfected with plasmids encoding for either Orai1α or Orai1β, we show that Orai1α plays a relevant role in agonist-induced NF-κB transcriptional activity. In contrast, functional Orai1β is not required for th

SUBMITTER: Nieto-Felipe J 

PROVIDER: S-EPMC9922819 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature