Brain blood vessel autoantibodies in patients with NMDA and GABA_A receptor encephalitis: identification of unconventional Myosin-X as target antigen.

ABSTRACT: Introduction: The antibody repertoire from CSF-derived antibody-secreting cells and memory B-cells in patients with encephalitis contains a considerable number of antibodies that do not target the disease-defining autoantigen such as the GABA or NMDA receptors. This study focuses on the functional relevance of autoantibodies to brain blood vessels in patients with GABA_A and NMDA receptor encephalitis. Methods: We tested 149 human monoclonal IgG antibodies from the cerebrospinal fluid of six patients with different forms of autoimmune encephalitis on murine brain sections for reactivity to blood vessels using immunohistochemistry. Positive candidates were tested for reactivity with purified brain blood vessels, effects on transendothelial electrical resistance (TEER

SUBMITTER: Li LY 

PROVIDER: S-EPMC9922905 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature