MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells.

ABSTRACT:

Background

The MUC1-C protein evolved in mammals to protect barrier tissues from loss of homeostasis; however, MUC1-C promotes oncogenesis in association with chronic inflammation. Aberrant expression of MUC1-C in cancers has been linked to depletion and dysfunction of T cells in the tumor microenvironment. In contrast, there is no known involvement of MUC1-C in the regulation of natural killer (NK) cell function.

Methods

Targeting MUC1-C genetically and pharmacologically in cancer cells was performed to assess effects on intracellular and cell surface expression of the MHC class I chain-related polypeptide A (MICA) and MICB ligands. The MICA/B promoters were analyzed for H3K27 and DNA methylation. Shedding of MICA/B was determined by ELISA. MUC1-C interactions with E

SUBMITTER: Morimoto Y 

PROVIDER: S-EPMC9923360 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature