Interaction between cucumber green mottle mosaic virus MP and CP promotes virus systemic infection.

ABSTRACT: The movement protein (MP) and coat protein (CP) of tobamoviruses play critical roles in viral cell-to-cell and long-distance movement, respectively. Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus. The functions of CGMMV MP and CP during viral infection remain largely unclear. Here, we show that CGMMV MP can interact with CP in vivo, and the amino acids at positions 79-128 in MP are vital for the MP-CP interaction. To confirm this finding, we mutated five conserved residues within the residue 79-128 region and six other conserved residues flanking this region, followed by in vivo interaction assays. The results showed that the conserved threonine residue at the position 107 in MP (MP^T107 ) is important for the MP-CP interaction. Substitution of T1

SUBMITTER: Shi YJ 

PROVIDER: S-EPMC9923391 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature