Biological Properties of Heparins Modified with an Arylazopyrazole-Based Photoswitch.

ABSTRACT: Unfractionated heparin (UFH) and enoxaparin (Enox) were substituted with a photoswitch (PS) showing quantitative trans-cis and cis-trans photoisomerizations. Long half-life of the cis photoisomer enabled comparison of the properties of heparins substituted with both PS photoisomers. Hydrodynamic diameter, D_h, of UFH-PS decreased upon trans-cis photoisomerization, the change being more pronounced for UFH-PS with a higher degree of substitution (DS), while D_h of Enox-PS did not significantly change. The anticoagulative properties of substituted heparins were significantly attenuated compared to non-substituted compounds. The interaction of UFH-PS with HSA, lysozyme, and protamine was studied with ITC. Under serum-free conditions

SUBMITTER: Stolarek M 

PROVIDER: S-EPMC9923745 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature