A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus.

ABSTRACT:

Background

Noroviruses are the most common cause of viral gastroenteritis worldwide, yet there is a deficit in the understanding of protective immunity. Surrogate neutralization assays have been widely used that measure the ability of antibodies to block virus-like particle (VLP) binding to histo-blood group antigens (HBGAs). However, screening large sample sets against multiple antigens using the traditional HBGA blocking assay requires significant investment in terms of time, equipment, and technical expertise, largely associated with the generation of purified VLPs.

Methods

To address these issues, a luciferase immunoprecipitation system (LIPS) assay was modified to measure the norovirus-specific HBGA blockade activity of antibodies. The assay (designated LIPS-Blockade) w

SUBMITTER: van Loben Sels JM 

PROVIDER: S-EPMC9924141 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature