Effect of Magnetic Microparticles on Cultivated Human Corneal Endothelial Cells.

ABSTRACT:

Purpose

To investigate effects of magnetic microparticles on movement of magnet controlled human corneal endothelial cells (HCECs).

Methods

Immortalized HCEC line (B4G12) and primary culture of HCECs were exposed to two commercially available magnetic micro- or nanoparticles, SiMAG (average size 100 nm) and fluidMAG (average size <1000 nm). Cell viability assays and reactive oxygen species production assays were performed. Cellular structural changes, intracellular distribution of microparticles, and expression levels of proteins related to cellular survival were analyzed. Ex vivo human corneas were exposed to microparticles to further evaluate their effects. Magnetic particle-laden HCECs were cultured under the influence of a neodymium magnet.

Results

No significant

SUBMITTER: Park JH 

PROVIDER: S-EPMC9924430 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature