Multi-target regulation of pro-inflammatory cytokine production by transcription factor Blimp-1.

ABSTRACT:

Objective

Cytokine storm syndrome is a fatal condition related to infectious and autoimmune diseases. Here, we aim to investigate the regulatory mechanisms of Blimp-1 on multiple cytokine production.

Methods

The Blimp1 shRNA was transfected into RAW264.7 macrophages, followed by Toll-like receptor (TLR) ligand stimulation. The mRNA and protein levels of cytokines were detected by real-time PCR and flow cytometric bead array. The nuclear translocation of AP-1 and NF-κB p65 was measured by immunofluorescence staining. The transcriptional activity was detected by luciferase reporter assay with 5 × NF-κB reporter or with IL6 promoter reporter.

Results

Blimp-1 significantly inhibited the expression and secretion of IL-1β, IL-6, and IL-18 in macrophages during stimulation w

SUBMITTER: Qin Q 

PROVIDER: S-EPMC9925500 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature