Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana.

ABSTRACT: Studying the interaction between the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and Arabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched from P. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF-MS. Additionally, fluorescently labelled N-glycans were quantified via HPLC-FLD. N-glycans from infected plants were overall less processed and displayed increased amounts of oligomannosidic N-glyc

SUBMITTER: Beihammer G 

PROVIDER: S-EPMC9925501 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature