Persistent homology analysis distinguishes pathological bone microstructure in non-linear microscopy images.

ABSTRACT: We present a topological method for the detection and quantification of bone microstructure from non-linear microscopy images. Specifically, we analyse second harmonic generation (SHG) and two photon excited autofluorescence (TPaF) images of bone tissue which capture the distribution of matrix (fibrillar collagen) structure and autofluorescent molecules, respectively. Using persistent homology statistics with a signed Euclidean distance transform filtration on binary patches of images, we are able to quantify the number, size, distribution, and crowding of holes within and across samples imaged at the microscale. We apply our methodology to a previously characterized murine model of skeletal pathology whereby vascular endothelial growth factor expression was deleted in osteocalcin-expressi

SUBMITTER: Pritchard Y 

PROVIDER: S-EPMC9925777 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature