ARMS-NF-κB signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress.

ABSTRACT: Ankyrin repeat-rich membrane spanning (ARMS) plays roles in neural development, neuropathies, and tumor formation. Such pleiotropic function of ARMS is often attributed to diverse ARMS-interacting molecules in different cell context. However, it might be achieved by ARMS' effect on global biological mediator like reactive oxygen species (ROS). We established ARMS-knockdown in melanoma cells (siARMS) and in Drosophila eyes (GMR>dARMS ^RNAi ) and challenged them with H₂O₂. Decreased ARMS in both systems compromises nuclear translocation of NF-κB and induces ROS, which in turn augments autophagy flux and confers susceptibility to H₂O₂-triggered autophagic cell death. Resuming NF-κB activity or reducing RO

SUBMITTER: Liao YH 

PROVIDER: S-EPMC9926119 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature