Deeply functional identification of TCS1 alleles provides efficient technical paths for low-caffeine breeding of tea plants.

ABSTRACT: Caffeine is an important functional component in tea, which has the effect of excitement and nerve stimulation, but excessive intake can cause insomnia and dysphoria. Therefore, the production of tea with low-caffeine content can meet the consumption needs of certain people. Here, in addition to the previous alleles of the tea caffeine synthase (TCS1) gene, a new allele (TCS1h) from tea germplasms was identified. Results of in vitro activity analysis showed that TCS1h had both theobromine synthase (TS) and caffeine synthase (CS) activities. Site-directed mutagenesis experiments of TCS1a, TCS1c, and TCS1h demonstrated that apart from the 225th amino acid residue, the 269th amino acid also determined the CS activity. GUS histochemical analysis and du

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9926157 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature