Structural and functional integration of human forebrain organoids with the injured adult rat visual system.

ABSTRACT: Brain organoids created from human pluripotent stem cells represent a promising approach for brain repair. They acquire many structural features of the brain and raise the possibility of patient-matched repair. Whether these entities can integrate with host brain networks in the context of the injured adult mammalian brain is not well established. Here, we provide structural and functional evidence that human brain organoids successfully integrate with the adult rat visual system after transplantation into large injury cavities in the visual cortex. Virus-based trans-synaptic tracing reveals a polysynaptic pathway between organoid neurons and the host retina and reciprocal connectivity between the graft and other regions of the visual system. Visual stimulation of host animals elicits resp

SUBMITTER: Jgamadze D 

PROVIDER: S-EPMC9926224 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature