Elevated human placental heat shock protein 5 is associated with spontaneous preterm birth.

ABSTRACT:

Background

Specific heat shock proteins are associated with pregnancy complications, including spontaneous preterm birth (SPTB). Placental proteomics and whole exome sequencing recently suggested an association between heat shock protein HSPA5 and uncomplicated SPTB. In the present study, we investigated the localization of and possible roles for HSPA5 in SPTB.

Methods

Western blot was performed to validate the result from the previously published proteomic analysis. We used qPCR to assess mRNA expression of genes and immunohistochemistry and immunoelectron microscopy to examine localization of HSPA5 in placental tissue. We silenced the HSPA5 gene in the HTR8/SVneo human trophoblast cell line to investigate possible functions of HSPA5.

Results

HSPA5 was upregulated in

SUBMITTER: Tissarinen P 

PROVIDER: S-EPMC9926443 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature