Transcriptomic, proteomic, and functional consequences of codon usage bias in human cells during heterologous gene expression.

ABSTRACT: Differences in codon frequency between genomes, genes, or positions along a gene, modulate transcription and translation efficiency, leading to phenotypic and functional differences. Here, we present a multiscale analysis of the effects of synonymous codon recoding during heterologous gene expression in human cells, quantifying the phenotypic consequences of codon usage bias at different molecular and cellular levels, with an emphasis on translation elongation. Six synonymous versions of an antibiotic resistance gene were generated, fused to a fluorescent reporter, and independently expressed in HEK293 cells. Multiscale phenotype was analyzed by means of quantitative transcriptome and proteome assessment, as proxies for gene expression; cellular fluorescence, as a proxy for single-cell lev

SUBMITTER: Picard MAL 

PROVIDER: S-EPMC9926478 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature