Dual Action of Eeyarestatin 24 on Sec-Dependent Protein Secretion and Bacterial DNA.

ABSTRACT: Eeyarestatin 24 (ES24) is a promising new antibiotic with broad-spectrum activity. It shares structural similarity with nitrofurantoin (NFT), yet appears to have a distinct and novel mechanism: ES24 was found to inhibit SecYEG-mediated protein transport and membrane insertion in Gram-negative bacteria. However, possible additional targets have not yet been explored. Moreover, its activity was notably better against Gram-positive bacteria, for which its mechanism of action had not yet been investigated. We have used transcriptomic stress response profiling, phenotypic assays, and protein secretion analyses to investigate the mode of action of ES24 in comparison with NFT using the Gram-positive model bacterium Bacillus subtilis and have compared our findings to Gram-negative Escher

SUBMITTER: Schafer AB 

PROVIDER: S-EPMC9926488 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature