AZD6738 promotes the tumor suppressive effects of trifluridine in colorectal cancer cells.

ABSTRACT: Ataxia telangiectasia and Rad3‑related (ATR) is a kinase that repairs DNA damage. Although inhibitors that selectively target ATR have been developed, their effectiveness in colorectal cancer has not been widely reported. The present study hypothesized that anticancer agents that effectively act in the S phase before the G₂/M checkpoint may be ideal agents for concomitant use with ATR inhibitors, which act at the G₂/M checkpoint. Therefore, the present study examined the combined effects of AZD6738, an ATR inhibitor, and trifluridine (FTD), which acts in the S phase and has a high DNA uptake rate. In vitro cell viability assays, flow cytometry and western blotting were performed to evaluate cell viability, and changes in cell cycle localization and protein expr

SUBMITTER: Harata S 

PROVIDER: S-EPMC9926513 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature