Single-copy Snail upregulation causes partial epithelial-mesenchymal transition in colon cancer cells.

ABSTRACT:

Background

Epithelial-mesenchymal transition (EMT) is an embryonic programme implicated in cancer stem cells, metastasis and therapeutic resistance. Its role in cancer progression remains controversial because the transition can be partial or complete in different models and contexts.

Methods

Using human colon cancer DLD-1 cells, we engineered a cell line with a single-copy of Snail that was doxycycline-inducible and compared it to existing EMT models in DLD-1. The effect of Snail upregulation was characterised functionally, morphologically, and by transcriptional profiling and protein expression.

Results

Induction with doxycycline increased Snail expression to a level similar to that observed in cancer cell lines spontaneously expressing Snail and results in partial

SUBMITTER: Junaid F 

PROVIDER: S-EPMC9926732 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature