Single-Cell Approach Reveals Intercellular Heterogeneity in Phage-Producing Capacities.

ABSTRACT: Bacteriophage burst size is the average number of phage virions released from infected bacterial cells, and its magnitude depends on the duration of an intracellular progeny accumulation phase. Burst size is often measured at the population level, not the single-cell level, and consequently, statistical moments are not commonly available. In this study, we estimated the bacteriophage lambda (λ) single-cell burst size mean and variance following different intracellular accumulation period durations by employing Escherichia coli lysogens bearing lysis-deficient λ prophages. Single lysogens can be isolated and chemically lysed at desired times following prophage induction to quantify progeny intracellular accumulation within individual cells. Our data showed that λ phage burst size initially

SUBMITTER: Kannoly S 

PROVIDER: S-EPMC9927085 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature