Mechanism of Action of Isopropoxy Benzene Guanidine against Multidrug-Resistant Pathogens.

ABSTRACT: The increasing emergence of antibiotic resistance is an urgent threat to global health care; thus, there is a need for new therapeutics. Guanidine is the preferred functional group for antimicrobial design and development. Herein, the potential antibacterial activity of the guanidine derivative isopropoxy benzene guanidine (IBG) against multidrug-resistant (MDR) bacteria was discovered. The synergistic antibacterial activity of IBG and colistin was determined by checkerboard assay, time-killing curve, and mouse experiments. The antibacterial mechanism of IBG was verified in fluorescent probe experiments, intracellular oxidative phosphorylation assays, and transcriptome analysis. The results showed that IBG displays efficient antibacterial activity against Gram-positive pathogens and Gram-n

SUBMITTER: Li J 

PROVIDER: S-EPMC9927234 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature