In vivo overexpression of synaptogyrin-3 promotes striatal synaptic dopamine uptake in LRRK2^R1441G  mutant mouse model of Parkinson's disease.

ABSTRACT:

Background

Leucine-rich repeat kinase 2 (LRRK2) mutation is a common genetic risk factor of Parkinson's disease (PD). Presynaptic dysfunction is an early pathogenic event associated with dopamine (DA) dysregulation in striatum of the brain. DA uptake activity of DA uptake transporter (DAT) affects synaptic plasticity and motor and non-motor behavior. Synaptogyrin-3 (SYNGR3) is part of the synaptogyrin family, especially abundant in brain. Previous in vitro studies demonstrated interaction between SYNGR3 and DAT. Reduced SYNGR3 expression was observed in human PD brains with unclear reasons.

Methods

Here, we further explored whether inducing SYNGR3 expression can influence (i) cellular DA uptake using differentiated human SH-SY5Y neuronal cells, (ii) striatal synaptosomal DA

SUBMITTER: Ho PW 

PROVIDER: S-EPMC9927849 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature