Patients with abdominal aortic aneurysms have reduced levels of microRNA 122-5p in circulating exosomes.

ABSTRACT:

Objective

There are currently no specific biomarkers to identify patients with abdominal aortic aneurysms (AAAs). Circulating exosomes contain microRNAs (miRNA) that are potential biomarkers for the presence of disease. This study aimed to characterize the exosomal miRNA expression profile of patients with AAAs in order to identify novel biomarkers of disease.

Methods

Patients undergoing duplex ultrasound (US) or computed tomography (CT) for screening or surveillance of an AAA were screened to participate in the study. Cases with AAA were defined as having a max aortic diameter >3 cm. Circulating plasma exosomes were isolated using Cushioned-Density Gradient Ultracentrifugation and total RNA was extracted. Next Generation Sequencing was performed on the Illumina HiSeq4000 SE

SUBMITTER: Lopez JL 

PROVIDER: S-EPMC9928131 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature