Neuroprotective effects of a lead compound from coral via modulation of the orphan nuclear receptor Nurr1.

ABSTRACT:

Aims

To screen coral-derived compounds with neuroprotective activity and clarify the potential mechanism of lead compounds.

Methods

The lead compounds with neuroprotective effects were screened by H₂ O₂ and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPP⁺ )-induced cell damage models in SH-SY5Y cells. CCK8 and LDH assays were used to detect cell viability. The anti-apoptosis of lead compounds was evaluated by flow cytometry. JC-1 and MitoSox assays were performed to examine the changes in mitochondrial membrane potential and mitochondrial ROS level. Survival of primary cortical and dopaminergic midbrain neurons was measured by MAP2 and TH immunoreactivities. The Caenorhabditis elegans (C. elegans) model was established to determine the effe

SUBMITTER: Su JW 

PROVIDER: S-EPMC9928544 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature