A new ¹³C trityl-based spin label enables the use of DEER for distance measurements.

ABSTRACT: Triarylmethyl (TAM)-based labels, while still underutilized, are a powerful class of labels for pulsed-Electron Spin Resonance (ESR) distance measurements. They feature slow relaxation rates for long-lasting signals, high stability for cellular experiments, and narrow spectral features for efficient excitation of the spins. However, the typical narrow line shape limits the available distance measurements to only single-frequency experiments, such as Double Quantum Coherence (DQC) and Relaxation Induced Dipolar Modulation Enhancement (RIDME), which can be complicated to perform or hard to process. Therefore, widespread usage of TAM labels can be enhanced by the use of Double Electron-Electron Resonance (DEER) distance measurements. In this work, we developed a new spin label, ¹³C

SUBMITTER: Hasanbasri Z 

PROVIDER: S-EPMC9928843 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature