In vitro assessment of Neuronal PAS domain 2 mitigating compounds for scarless wound healing.

ABSTRACT:

Background

The core circadian gene Neuronal PAS domain 2 (NPAS2) is expressed in dermal fibroblasts and has been shown to play a critical role in regulating collagen synthesis during wound healing. We have performed high throughput drug screening to identify genes responsible for downregulation of Npas2 while maintaining cell viability. From this, five FDA-approved hit compounds were shown to suppress Npas2 expression in fibroblasts. In this study, we hypothesize that the therapeutic suppression of Npas2 by hit compounds will have two effects: (1) attenuated excessive collagen deposition and (2) accelerated dermal wound healing without hypertrophic scarring.

Materials and methods

To test the effects of each hit compound (named Dwn1, 2, 3, 4, and 5

SUBMITTER: Clements A 

PROVIDER: S-EPMC9928850 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature