Blood Milieu in Acute Myocardial Infarction Reprograms Human Macrophages for Trauma Repair.

ABSTRACT: Acute myocardial infarction (AMI) is accompanied by a systemic trauma response that impacts the whole body, including blood. This study addresses whether macrophages, key players in trauma repair, sense and respond to these changes. For this, healthy human monocyte-derived macrophages are exposed to 20% human AMI (n = 50) or control (n = 20) serum and analyzed by transcriptional and multiparameter functional screening followed by network-guided data interpretation and drug repurposing. Results are validated in an independent cohort at functional level (n = 47 AMI, n = 25 control) and in a public dataset. AMI serum exposure results in an overt AMI signature, enriched in debris cleaning, mitosis, and immune pathways. Moreover, gene networks associated with AMI and with poor clinical prognosi

SUBMITTER: Fontaine MAC 

PROVIDER: S-EPMC9929255 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature