Exosomes are involved in iron transport from human blood-brain barrier endothelial cells and are modified by endothelial cell iron status.

ABSTRACT: Iron is essential for normal brain development and function. Hence, understanding the mechanisms of iron efflux at the blood-brain barrier and their regulation are critical for the establishment of brain iron homeostasis. Here, we have investigated the role of exosomes in mediating the transfer of H-ferritin (FTH1)- or transferrin (Tf)-bound iron across the blood-brain barrier endothelial cells (BBBECs). Our study used ECs derived from human-induced pluripotent stem cells that are grown in bicameral chambers. When cells were exposed to ⁵⁵Fe-Tf or ⁵⁵Fe-FTH1, the ⁵⁵Fe activity in the exosome fraction in the basal chamber was significantly higher compared to the supernatant fraction. Furthermore, we determined that the release of endogenous Tf, FTH1, and exoso

SUBMITTER: Palsa K 

PROVIDER: S-EPMC9929479 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature