Proteoforms expand the world of microproteins and short open reading frame-encoded peptides.

ABSTRACT: Microproteins and short open reading frame-encoded peptides (SEPs) can, like all proteins, carry numerous posttranslational modifications. Together with posttranscriptional processes, this leads to a high number of possible distinct protein molecules, the proteoforms, out of a limited number of genes. The identification, quantification, and molecular characterization of proteoforms possess special challenges to established, mainly bottom-up proteomics (BUP) based analytical approaches. While BUP methods are powerful, proteins have to be inferred rather than directly identified, which hampers the detection of proteoforms. An alternative approach is top-down proteomics (TDP) which allows to identify intact proteoforms. This perspective article provides a brief overview of modified microprote

SUBMITTER: Cassidy L 

PROVIDER: S-EPMC9929600 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature