A novel human multiple myeloma cell line with a 1q21 gain genetic abnormality and CKS1B overexpression.

ABSTRACT:

Background

Multiple myeloma (MM) is an incurable hematologic malignancy mainly due to its cytogenetic abnormalities. Therefore, it is important to establish permanent malignant MM cell lines as tools to develop more effective therapies.

Methods

Pleural effusion cells of a 70-year-old patient was collected to establish the CZ2 cell line. Characterization of CZ2 was determined with nephelometry, flow cytometry, fluorescence in situ hybridization (FISH), and chromosomal microarray analysis (CMA). Western blotting analysis was adopted to determine protein expression. Cell viability was measured by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.

Results

We established and characterized a new MM cell line, CZ2. Using nephelometry and flow cyt

SUBMITTER: He J 

PROVIDER: S-EPMC9929770 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature