The central glial cell line-derived neurotrophic factor (GDNF) regulates pulmonary function in asthmatic rats.

ABSTRACT:

Background

Asthma is a common chronic inflammatory disease of the airway, but the mechanism is still not fully understood. This study aimed to investigate the effect of glial cell line-derived neurotrophic factor (GDNF) on asthma attacks.

Methods

An asthmatic rat model was established. GDNF expression in the airway and brain was observed by immunohistochemistry (IHC), and the concentration of GDNF in bronchoalveolar lavage fluid (BALF) was detected by enzyme-linked immunosorbent assay (ELISA). After injection of GDNF and its antibody into the lateral ventricle of asthmatic rats, the pulmonary function was recorded, and the levels of interferon-γ (IFN-γ) and interleukin-4 (IL-4) in BALF were tested.

Results

GDNF expressions were increased significantly in the lung tiss

SUBMITTER: Wang X 

PROVIDER: S-EPMC9929813 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature