Optimization of Media Change Intervals through Hydrogels Using Mathematical Models.

ABSTRACT: Three-dimensional cell culture in engineered hydrogels is increasingly used in tissue engineering and regenerative medicine. The transfer of nutrients, gases, and waste materials through these hydrogels is of utmost importance for cell viability and response, yet the translation of diffusion coefficients into practical guidelines is not well established. Here, we combined mathematical modeling, fluorescent recovery after photobleaching, and hydrogel diffusion experiments on cell culture inserts to provide a multiscale practical approach for diffusion. We observed a dampening effect of the hydrogel that slowed the response to concentration changes and the creation of a diffusion gradient in the hydrogel by media refreshment. Our designed model combined with measurements provides a practical

SUBMITTER: Ruiter FAA 

PROVIDER: S-EPMC9930106 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature