Microscopic mechanism of PIEZO1 activation by pressure-induced membrane stretch.

ABSTRACT: Mechanosensitive PIEZO1 ion channels open in response to membrane stretch. Yet, the underlying microscopic mechanism of this activation remains unknown. To probe this mechanism, we used cell-attached pressure-clamp recordings to measure single channel currents at different steady-state negative pipette pressures, spanning the full range of the channel's pressure sensitivity. Pressure-dependent activation occurs through a sharp reduction of the mean shut duration and through a moderate increase of the mean open duration. Across all tested pressures, the distribution of open and shut dwell times best follows sums of two and three exponential components, respectively. As the magnitude of the pressure stimulus increases, the time constants of most of these exponential components gradually chan

SUBMITTER: Wijerathne TD 

PROVIDER: S-EPMC9930135 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature