Cardiomyocyte sarcomere length variability: Membrane fluorescence versus second harmonic generation myosin imaging.

ABSTRACT: Sarcomere length (SL) and its variation along the myofibril strongly regulate integrated coordinated myocyte contraction. It is therefore important to obtain individual SL properties. Optical imaging by confocal fluorescence (for example, using ANEPPS) or transmitted light microscopy is often used for this purpose. However, this allows for the visualization of structures related to Z-disks only. In contrast, second-harmonic generation (SHG) microscopy visualizes A-band sarcomeric structures directly. Here, we compared averaged SL and its variability in isolated relaxed rat cardiomyocytes by imaging with ANEPPS and SHG. We found that SL variability, evaluated by several absolute and relative measures, is two times smaller using SHG vs. ANEPPS, while both optical methods give the same averag

SUBMITTER: Lookin O 

PROVIDER: S-EPMC9930136 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature