TLR4 mediates alveolar bone resorption in experimental peri-implantitis through regulation of CD45⁺ cell infiltration, RANKL/OPG ratio, and inflammatory cytokine production.

ABSTRACT:

Background

The present study was to determine the role of Toll-like receptor 4 (TLR4) signaling in inflammation and alveolar bone resorption using a murine model of Porphyromonas gingivalis-associated ligature-induced peri-implantitis.

Methods

Smooth surface titanium implants were placed in the left maxilla alveolar bone 6 weeks after extraction of first and second molars in Wild-type (WT) and TLR4^-/- (TLR4 KO) mice. Silk ligatures immersed with P. gingivalis were tied around the implants 4 weeks after the implant placement and confirmation of osteointegration. Two weeks after the ligation, bone resorption, osteoclastogenesis, cellular inflammatory responses, and gingival mRNA expression levels of cytokines were assessed by micro-computed tomography, tartrate-resi

SUBMITTER: Deng S 

PROVIDER: S-EPMC9930181 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature