Reducing bias and variance for CTF estimation in single particle cryo-EM.

ABSTRACT: When using an electron microscope for imaging of particles embedded in vitreous ice, the recorded image, or micrograph, is a significantly degraded version of the tomographic projection of the sample. Apart from noise, the image is affected by the optical configuration of the microscope. This transformation is typically modeled as a convolution with a point spread function. The Fourier transform of this function, known as the contrast transfer function (CTF), is oscillatory, attenuating and amplifying different frequency bands, and sometimes flipping their signs. High-resolution reconstruction requires this CTF to be accounted for, but as its form depends on experimental parameters, it must first be estimated from the micrograph. We present a new method for CTF estimation based on multitap

SUBMITTER: Heimowitz A 

PROVIDER: S-EPMC9930185 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature