Regulon active landscape reveals cell development and functional state changes of human primary osteoblasts in vivo.

ABSTRACT:

Background

While transcription factor (TF) regulation is known to play an important role in osteoblast development, differentiation, and bone metabolism, the molecular features of TFs in human osteoblasts at the single-cell resolution level have not yet been characterized. Here, we identified modules (regulons) of co-regulated genes by applying single-cell regulatory network inference and clustering to the single-cell RNA sequencing profiles of human osteoblasts. We also performed cell-specific network (CSN) analysis, reconstructed regulon activity-based osteoblast development trajectories, and validated the functions of important regulons both in vivo and in vitro.

Results

We identified four cell clusters: preosteoblast-S1, preosteoblast-S2, intermediate osteoblasts, and ma

SUBMITTER: Wang S 

PROVIDER: S-EPMC9930257 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature