Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.

ABSTRACT:

Background

Agrobacterium tumefaciens-mediated leaf disc genetic transformation is an important way to achieve transgenics or gene editing. Ensuring stable and efficient genetic transformation is still an important problem in modern biology. It is assumed that the difference in the development status of genetic transformation cells of receptor materials is the main reason for the difference and instability of genetic transformation efficiency; the stable and efficient genetic transformation rate can be obtained by defining the appropriate treatment period of the receptor material and applying genetic transformation in a timely manner.

Results

Based on these assumptions, we studied and established an efficient and stable Agrobacterium-mediated plant transformation system with

SUBMITTER: Xia Y 

PROVIDER: S-EPMC9930321 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature