Development of a TaqMan qPCR assay for trypanosomatid multi-species detection and quantification in insects.

ABSTRACT:

Background

Trypanosomatid parasites are widely distributed in nature and can have a monoxenous or dixenous life-cycle. These parasites thrive in a wide number of insect orders, some of which have an important economic and environmental value, such as bees. The objective of this study was to develop a robust and sensitive real-time quantitative PCR (qPCR) assay for detecting trypanosomatid parasites in any type of parasitized insect sample.

Methods

A TaqMan qPCR assay based on a trypanosomatid-conserved region of the α-tubulin gene was standardized and evaluated. The limits of detection, sensitivity and versatility of the α-tubulin TaqMan assay were tested and validated using field samples of honeybee workers, wild bees, bumblebees and grasshoppers, as well as in the human in

SUBMITTER: Barranco-Gomez O 

PROVIDER: S-EPMC9930332 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature